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Pyle April

Title(s)Associate Professor, Microbiology Immunology & Molecular Genetics
Phone310-794-4059
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    Collapse Research 
    Collapse Research Activities and Funding
    Reprogramming and Directed Differentiation of Skeletal Muscle Cells from hPSCs.
    NIH/NIAMS R01AR064327Sep 20, 2013 - Nov 30, 2023
    Role: Principal Investigator
    Development of High Content Screens for Human Pluripotent Stem Cells
    NIH/NINDS R21NS064855Sep 30, 2008 - Aug 31, 2012
    Role: Principal Investigator
    Control of Stem Cell Fate in Heart Coculture
    NIH/NHLBI F32HL074596Aug 1, 2003 - Jul 31, 2005
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Young CS, Pyle AD, Spencer MJ. CRISPR for Neuromuscular Disorders: Gene Editing and Beyond. Physiology (Bethesda). 2019 Sep 01; 34(5):341-353. PMID: 31389773.
      View in: PubMed
    2. Hazim RA, Karumbayaram S, Jiang M, Dimashkie A, Lopes VS, Li D, Burgess BL, Vijayaraj P, Alva-Ornelas JA, Zack JA, Kohn DB, Gomperts BN, Pyle AD, Lowry WE, Williams DS. Correction to: Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Res Ther. 2019 Feb 12; 10(1):52. PMID: 30755264.
      View in: PubMed
    3. Ferguson GB, Van Handel B, Bay M, Fiziev P, Org T, Lee S, Shkhyan R, Banks NW, Scheinberg M, Wu L, Saitta B, Elphingstone J, Larson AN, Riester SM, Pyle AD, Bernthal NM, Mikkola HK, Ernst J, van Wijnen AJ, Bonaguidi M, Evseenko D. Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes. Nat Commun. 2018 09 07; 9(1):3634. PMID: 30194383.
      View in: PubMed
    4. Hicks MR, Hiserodt J, Paras K, Fujiwara W, Eskin A, Jan M, Xi H, Young CS, Evseenko D, Nelson SF, Spencer MJ, Handel BV, Pyle AD. ERBB3 and NGFR mark a distinct skeletal muscle progenitor cell in human development and hPSCs. Nat Cell Biol. 2018 01; 20(1):46-57. PMID: 29255171.
      View in: PubMed
    5. Hazim RA, Karumbayaram S, Jiang M, Dimashkie A, Lopes VS, Li D, Burgess BL, Vijayaraj P, Alva-Ornelas JA, Zack JA, Kohn DB, Gomperts BN, Pyle AD, Lowry WE, Williams DS. Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Res Ther. 2017 10 02; 8(1):217. PMID: 28969679.
      View in: PubMed
    6. Xi H, Fujiwara W, Gonzalez K, Jan M, Liebscher S, Van Handel B, Schenke-Layland K, Pyle AD. In Vivo Human Somitogenesis Guides Somite Development from hPSCs. Cell Rep. 2017 02 07; 18(6):1573-1585. PMID: 28178531.
      View in: PubMed
    7. Young CS, Mokhonova E, Quinonez M, Pyle AD, Spencer MJ. Creation of a Novel Humanized Dystrophic Mouse Model of Duchenne Muscular Dystrophy and Application of a CRISPR/Cas9 Gene Editing Therapy. J Neuromuscul Dis. 2017; 4(2):139-145. PMID: 28505980.
      View in: PubMed
    8. Lee PC, Truong B, Vega-Crespo A, Gilmore WB, Hermann K, Angarita SA, Tang JK, Chang KM, Wininger AE, Lam AK, Schoenberg BE, Cederbaum SD, Pyle AD, Byrne JA, Lipshutz GS. Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells. Mol Ther Nucleic Acids. 2016 Nov 29; 5(11):e394. PMID: 27898091.
      View in: PubMed
    9. Young CS, Pyle AD. Exon Skipping Therapy. Cell. 2016 Nov 17; 167(5):1144. PMID: 27863231.
      View in: PubMed
    10. Young CS, Hicks MR, Ermolova NV, Nakano H, Jan M, Younesi S, Karumbayaram S, Kumagai-Cresse C, Wang D, Zack JA, Kohn DB, Nakano A, Nelson SF, Miceli MC, Spencer MJ, Pyle AD. A Single CRISPR-Cas9 Deletion Strategy that Targets the Majority of DMD Patients Restores Dystrophin Function in hiPSC-Derived Muscle Cells. Cell Stem Cell. 2016 Apr 07; 18(4):533-40. PMID: 26877224.
      View in: PubMed
    11. Thakore-Shah K, Koleilat T, Jan M, John A, Pyle AD. REST/NRSF Knockdown Alters Survival, Lineage Differentiation and Signaling in Human Embryonic Stem Cells. PLoS One. 2015; 10(12):e0145280. PMID: 26690059.
      View in: PubMed
    12. Hicks M, Pyle A. The Path from Pluripotency to Skeletal Muscle: Developmental Myogenesis Guides the Way. Cell Stem Cell. 2015 Sep 03; 17(3):255-7. PMID: 26340524.
      View in: PubMed
    13. Hou S, Choi JS, Chen KJ, Zhang Y, Peng J, Garcia MA, Yu JH, Thakore-Shah K, Ro T, Chen JF, Peyda P, Fan G, Pyle AD, Wang H, Tseng HR. Supramolecular nanosubstrate-mediated delivery for reprogramming and transdifferentiation of mammalian cells. Small. 2015 Jun 03; 11(21):2499-504. PMID: 25613059.
      View in: PubMed
    14. Peng J, Garcia MA, Choi JS, Zhao L, Chen KJ, Bernstein JR, Peyda P, Hsiao YS, Liu KW, Lin WY, Pyle AD, Wang H, Hou S, Tseng HR. Molecular recognition enables nanosubstrate-mediated delivery of gene-encapsulated nanoparticles with high efficiency. ACS Nano. 2014 May 27; 8(5):4621-9. PMID: 24708312.
      View in: PubMed
    15. Awe JP, Lee PC, Ramathal C, Vega-Crespo A, Durruthy-Durruthy J, Cooper A, Karumbayaram S, Lowry WE, Clark AT, Zack JA, Sebastiano V, Kohn DB, Pyle AD, Martin MG, Lipshutz GS, Phelps PE, Pera RA, Byrne JA. Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status. Stem Cell Res Ther. 2013 Jul 26; 4(4):87. PMID: 23890092.
      View in: PubMed
    16. Sherman SP, Pyle AD. Small molecule screening with laser cytometry can be used to identify pro-survival molecules in human embryonic stem cells. PLoS One. 2013; 8(1):e54948. PMID: 23383009.
      View in: PubMed
    17. Palomares K, Vigant F, Van Handel B, Pernet O, Chikere K, Hong P, Sherman SP, Patterson M, An DS, Lowry WE, Mikkola HK, Morizono K, Pyle AD, Lee B. Nipah virus envelope-pseudotyped lentiviruses efficiently target ephrinB2-positive stem cell populations in vitro and bypass the liver sink when administered in vivo. J Virol. 2013 Feb; 87(4):2094-108. PMID: 23192877.
      View in: PubMed
    18. Yanes RE, Tarn D, Hwang AA, Ferris DP, Sherman SP, Thomas CR, Lu J, Pyle AD, Zink JI, Tamanoi F. Involvement of lysosomal exocytosis in the excretion of mesoporous silica nanoparticles and enhancement of the drug delivery effect by exocytosis inhibition. Small. 2013 Mar 11; 9(5):697-704. PMID: 23152124.
      View in: PubMed
    19. Jonas SJ, Alva JA, Richardson W, Sherman SP, Galic Z, Pyle AD, Dunn B. A spatially and chemically defined platform for the uniform growth of human pluripotent stem cells. Mater Sci Eng C Mater Biol Appl. 2013 Jan 01; 33(1):234-41. PMID: 25428067.
      View in: PubMed
    20. Karumbayaram S, Lee P, Azghadi SF, Cooper AR, Patterson M, Kohn DB, Pyle A, Clark A, Byrne J, Zack JA, Plath K, Lowry WE. From skin biopsy to neurons through a pluripotent intermediate under Good Manufacturing Practice protocols. Stem Cells Transl Med. 2012 Jan; 1(1):36-43. PMID: 23197638.
      View in: PubMed
    21. Alva JA, Lee GE, Escobar EE, Pyle AD. Phosphatase and tensin homolog regulates the pluripotent state and lineage fate choice in human embryonic stem cells. Stem Cells. 2011 Dec; 29(12):1952-62. PMID: 21948699.
      View in: PubMed
    22. Le Belle JE, Orozco NM, Paucar AA, Saxe JP, Mottahedeh J, Pyle AD, Wu H, Kornblum HI. Proliferative neural stem cells have high endogenous ROS levels that regulate self-renewal and neurogenesis in a PI3K/Akt-dependant manner. Cell Stem Cell. 2011 Jan 07; 8(1):59-71. PMID: 21211782.
      View in: PubMed
    23. Sherman SP, Alva JA, Thakore-Shah K, Pyle AD. Human pluripotent stem cells: the development of high-content screening strategies. Methods Mol Biol. 2011; 767:283-95. PMID: 21822883.
      View in: PubMed
    24. Tchieu J, Kuoy E, Chin MH, Trinh H, Patterson M, Sherman SP, Aimiuwu O, Lindgren A, Hakimian S, Zack JA, Clark AT, Pyle AD, Lowry WE, Plath K. Female human iPSCs retain an inactive X chromosome. Cell Stem Cell. 2010 Sep 03; 7(3):329-42. PMID: 20727844.
      View in: PubMed
    25. Tseng HC, Arasteh A, Paranjpe A, Teruel A, Yang W, Behel A, Alva JA, Walter G, Head C, Ishikawa TO, Herschman HR, Cacalano N, Pyle AD, Park NH, Jewett A. Increased lysis of stem cells but not their differentiated cells by natural killer cells; de-differentiation or reprogramming activates NK cells. PLoS One. 2010 Jul 16; 5(7):e11590. PMID: 20661281.
      View in: PubMed
    26. Kamei K, Ohashi M, Gschweng E, Ho Q, Suh J, Tang J, For Yu ZT, Clark AT, Pyle AD, Teitell MA, Lee KB, Witte ON, Tseng HR. Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions. Lab Chip. 2010 May 07; 10(9):1113-9. PMID: 20390128.
      View in: PubMed
    27. Zeng W, de Greef JC, Chen YY, Chien R, Kong X, Gregson HC, Winokur ST, Pyle A, Robertson KD, Schmiesing JA, Kimonis VE, Balog J, Frants RR, Ball AR, Lock LF, Donovan PJ, van der Maarel SM, Yokomori K. Specific loss of histone H3 lysine 9 trimethylation and HP1gamma/cohesin binding at D4Z4 repeats is associated with facioscapulohumeral dystrophy (FSHD). PLoS Genet. 2009 Jul; 5(7):e1000559. PMID: 19593370.
      View in: PubMed
    28. Chin MH, Mason MJ, Xie W, Volinia S, Singer M, Peterson C, Ambartsumyan G, Aimiuwu O, Richter L, Zhang J, Khvorostov I, Ott V, Grunstein M, Lavon N, Benvenisty N, Croce CM, Clark AT, Baxter T, Pyle AD, Teitell MA, Pelegrini M, Plath K, Lowry WE. Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures. Cell Stem Cell. 2009 Jul 02; 5(1):111-23. PMID: 19570518.
      View in: PubMed
    29. Damoiseaux R, Sherman SP, Alva JA, Peterson C, Pyle AD. Integrated chemical genomics reveals modifiers of survival in human embryonic stem cells. Stem Cells. 2009 Mar; 27(3):533-542. PMID: 19074420.
      View in: PubMed
    30. Conway AE, Lindgren A, Galic Z, Pyle AD, Wu H, Zack JA, Pelligrini M, Teitell MA, Clark AT. A self-renewal program controls the expansion of genetically unstable cancer stem cells in pluripotent stem cell-derived tumors. Stem Cells. 2009 Jan; 27(1):18-28. PMID: 19224508.
      View in: PubMed
    31. Wu H, Kim KJ, Mehta K, Paxia S, Sundstrom A, Anantharaman T, Kuraishy AI, Doan T, Ghosh J, Pyle AD, Clark A, Lowry W, Fan G, Baxter T, Mishra B, Sun Y, Teitell MA. Copy number variant analysis of human embryonic stem cells. Stem Cells. 2008 Jun; 26(6):1484-9. PMID: 18369100.
      View in: PubMed
    32. Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells. Stem Cells. 2008 Jun; 26(6):1436-43. PMID: 18323409.
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    33. Pyle AD, Lock LF, Donovan PJ. Neurotrophins mediate human embryonic stem cell survival. Nat Biotechnol. 2006 Mar; 24(3):344-50. PMID: 16444268.
      View in: PubMed
    34. Fenderson BA, De Miguel MP, Pyle AD, Donovan PJ. Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens. Methods Mol Biol. 2006; 325:207-24. PMID: 16761728.
      View in: PubMed
    35. Dravid G, Ye Z, Hammond H, Chen G, Pyle A, Donovan P, Yu X, Cheng L. Defining the role of Wnt/beta-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells. Stem Cells. 2005 Nov-Dec; 23(10):1489-501. PMID: 16002782.
      View in: PubMed
    36. Pyle AD, Donovan PJ, Lock LF. Chipping away at 'stemness'. Genome Biol. 2004; 5(8):235. PMID: 15287966.
      View in: PubMed